SulfoLink Immobilization Kits and Coupling Resin
产品名称： SulfoLink Immobilization Kits and Coupling Resin
英文名称： SulfoLink Immobilization Kits and Coupling Resin
产品编号： 20325
产品价格： 0
产品产地： USA
品牌商标： Pierce
更新时间： 2024-12-10T13:25:09
使用范围： null
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- 邮编 : 200030
- 所在区域 : 上海
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SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups. When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2 ml of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.
General structure and reaction scheme for SulfoLink Resin
 Learn more about the SulfoLink Immobilization Kit for Peptides (Opens a new window) |
 Learn more about the SulfoLink Immobilization Kit for Proteins |
Highlights:
- Specific conjugation through sulfhydryl (-SH) groups – The iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
- Separate kits optimized for peptides or proteins – Kits include optimized reagents for preparing peptide or protein samples for efficient immobilization
- Fast – Spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
- Flexible coupling conditions – Use pH 7.5-9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine•HCl), as needed for protein or peptide solubility during coupling reaction
- Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization, and affinity purification
- High capacity – Immobilize 1-2 mg peptide or 2-20 mg protein per 2-ml column of SulfoLink Coupling Resin.
Applications:
- Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
- Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
Related Products:
Bond-Breaker TCEP Solution, Neutral pH Product # Description Pkg. Size SulfoLink Immobilization Kit for Proteins Kit SulfoLink Immobilization Kit for Peptides Kit 20325 SulfoLink Immobilization Trial Kit Kit 20401 SulfoLink Coupling Resin 10 ml 20402 SulfoLink Coupling Resin 50 ml 20404 SulfoLink Coupling Resin 250 ml
Imject Maleimide Activated mcKLH Kit
UltraLink Iodoacetyl Resin
Micro-scale Peptide Coupling Kit
AminoLink Plus Immobilization Kit
CarboLink Immobilization Kit
CarboxyLink Immobilization Kit
Pierce Centrifuge Columns
Sufficient reagents to prepare five affinity columns.
Kit contains:
SulfoLink Columns, 5 x 2 ml
SulfoLink Sample Preparation Buffer, 7.5 ml
SulfoLink Coupling Buffer, 500 ml
Wash Solution, 120 ml
2-Mercaptoethylamine•HCl, 5 x 6 mg
L-Cysteine•HCl, 100 mg
Zeba Desalt Spin Columns, 5 x 5 ml
Phosphate Buffered Saline, 1 Pack
Columns Accessories
Replaces product # 44895 for use with proteins
Sufficient reagents to prepare five affinity columns.
Kit contains:
SulfoLink Columns, 5 x 2 ml
SulfoLink Coupling Buffer, 120 ml
Wash Solution, 120 ml
Bond-Breaker TCEP Solution, 0.5 ml
L-Cysteine•HCl, 100 mg
Phosphate Buffered Saline, 1 Pack
Column Accessories
Replaces product # 44895 for use with peptides
Sufficient reagents to prepare 1 affinity column with a peptide or protein.
Kit contains:
SulfoLink Column, 1 x 2 ml
SulfoLink Sample Preparation Buffer, 7.5 ml
SulfoLink Coupling Buffer, 120 ml
Wash Solution, 25 ml
2-Mercaptoethylamine•HCl, 6 mg
Bond-Breaker TCEP Solution, 0.5 ml
Cysteine•HCl, 100 mg
Zeba Desalt Spin Column, 5 ml
Phosphate Buffered Saline, 1 Pack
Columns Accessories
Replaces product # 20405