Disease:

 Insulinoma (Radiation induced cell immortalization) 

Origin:

 Pancreatic Islets 

Species:

Rattus Norvegicus 

Tissue:

Pancreas 

Morphology:

β cells 

Properties:

Adherent 

Medium:

RPMI1640 + 10% FBS, 10 mM HEPES, 2 mM L-glutamine, 1x sodium pyruvate, 0.05 mM 2-mercaptoethanol 

Subculture:

1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Insulin Responsiveness:

Respond to glucose within the physiological range. Relatively high insulin content.

Related cells by Organ:

AR-42J cells 

BxPc-3 cells 

Capan-1 cells 

MIA PaCa-2 cells 

E3LZ10.7 cells 

A10.7 cells 

INS-1 cells 

MIN-6 cells 

INS-1E cells 


References for INS-1 cells:

1. Asfari, M., Janjic, D., Meda, P. et al. (1992). Establishment of 2-mercaptoethanol-dependent differentiated insulin- secreting cell lines. Endocrinology 130, 167-178.