RBM9 monoclonal antibody (M01), clone 4G3
产品名称： RBM9 monoclonal antibody (M01), clone 4G3
英文名称： RBM9 monoclonal antibody (M01), clone 4G3
产品编号： H00023543-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant RBM9.
- Immunogen:
- RBM9 (AAH13115, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MEKKKMVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQS
- Host:
- Mouse
- Reactivity:
- Human, Mouse
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- FOX-2 Dependent Splicing of Ataxin-2 Transcript Is Affected by Ataxin-1 Overexpression.
Welzel F, Kaehler C, Isau M, Hallen L, Lehrach H, Krobitsch S.PLoS One. 2012;7(5):e37985. Epub 2012 May 30.
- Applications
- Western Blot (Cell lysate)
- RBM9 monoclonal antibody (M01), clone 4G3 Western Blot analysis of RBM9 expression in NIH/3T3 ( Cat # L018V1 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibody (M01), clone 4G3.
Lane 1: RBM9 transfected lysate(40.4 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to RBM9 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged RBM9 is approximately 0.1ng/ml as a capture antibody.
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi ( Cat # H00023543-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody (M01), clone 4G3 (Cat # H00023543-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunofluorescence
- enlarge
- Sandwich ELISA (Recombinant protein)
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- Entrez GeneID:
- 23543
- GeneBank Accession#:
- BC013115
- Protein Accession#:
- AAH13115
- Gene Name:
- RBM9
- Gene Alias:
- FOX2,Fox-2,HNRBP2,HRNBP2,RTA,dJ106I20.3,fxh
- Gene Description:
- RNA binding motif protein 9
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- OTTHUMP00000028771,fox-1 homologue,hexaribonucleotide binding protein 2,repressor of tamoxifen transcriptional activity